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KMID : 0380019940090030294
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.294 ~ p.297
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Improved Recombinant β-Galactosidase production Using Medium Additives at NcNPV Infection of Insect Cells in Batch and Continuous Two-Stage Bioreators
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Kim, Jee Sun/김지선
Lee, Ki Woong/Kang, Seok Kwon/Yang, Jai Myung/Chung, In Sik/이기웅/강석권/양재명/정인식
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Abstract
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The medium additives such as CaCI;glucose, frutose, glutamine, glutamate and lipids were to enhance recombinant /3-galactosidase(/3-gal) production in batch and continuous two-stage bioreactor systems. The presence of each medium additive such as CaCl;fructose, glutamate, cholesterol and tocopherol at AcNPV infection of Sf 21 cells had an effect on improved /9-gal production. The recombinant /3- gal production using the infection media supplemented with a mixture of 30mM CaCI,, 2.2mM fructose, 4.1mM glutamate and 0.34mM cholesterol was increased by about 40%.
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